Mass Spectrometry and Peptide Analysis

Mass spectrometry measures ions by mass-to-charge ratio and can support peptide identity characterization when used with an appropriate method.

Identity versus purity

A mass spectrum can provide evidence related to molecular mass or ion pattern. It does not automatically describe every component in a sample or replace other analytical methods.

Read the method notes

Interpretation depends on ionization mode, instrument configuration, calibration, sample preparation, and the reported acceptance criteria. A useful report identifies the sample and method clearly.

Pairing methods

Laboratories may use chromatography and mass spectrometry together because separation and mass-based detection answer related but different analytical questions.

Reference

Analytical procedures for peptide quantification by LC-MS, PubMed.